Goat F(ab) Anti-Rat IgG H&L (Texas Red ®) (ab7175)

Overview

  • Product nameGoat F(ab) Anti-Rat IgG H&L (Texas Red ®)See all IgG secondary antibodies ...
  • Description
    Goat F(ab) polyclonal Secondary Antibody to Rat IgG - H&L (TR)
  • Target speciesRat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationTexas Red ®. Ex: 596nm, Em: 620nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.0 moles Texas Red per mole of Goat IgG Fab
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
ELISA
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab) Anti-Rat IgG H&L (Texas Red ®) (ab7175)

    ab7175 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"