Goat F(ab) Anti-Rat IgG H&L (TRITC) (ab7174)

Overview

  • Product name
    Goat F(ab) Anti-Rat IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, Immunomicroscopymore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.0 moles TRITC per mole of Goat IgG Fab
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Flow Cyt 1/2000 - 1/10000.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab) Anti-Rat IgG H&L (TRITC) (ab7174)

ab7174 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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