Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

Overview

  • Product nameGoat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: Flow Cytmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

References for Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

ab98596 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5ug/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
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Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"