Goat F(ab')2 Anti-Human IgG H&L (HRP) (ab98525)
Key features and details
- Goat F(ab')2 Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
Overview
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Product name
Goat F(ab')2 Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: ICC, ELISA, IHC-P, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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ELISA |
1/10000 - 1/100000. as primary
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IHC-P |
1/200 - 1/5000.
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WB |
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
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Notes |
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ICC
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/100000. as primary |
IHC-P
1/200 - 1/5000. |
WB
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab98525 has been referenced in 1 publication.
- Burvenich IJ et al. Cross-species analysis of Fc engineered anti-Lewis-Y human IgG1 variants in human neonatal receptor transgenic mice reveal importance of S254 and Y436 in binding human neonatal Fc receptor. MAbs 8:775-86 (0). PubMed: 27030023