This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/10000 - 1/100000. as primary
1/200 - 1/5000.
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
References for Goat F(ab')2 Anti-Human IgG H&L (HRP) (ab98525)
This product has been referenced in:
Burvenich IJ et al. Cross-species analysis of Fc engineered anti-Lewis-Y human IgG1 variants in human neonatal receptor transgenic mice reveal importance of S254 and Y436 in binding human neonatal Fc receptor. MAbs8:775-86 (0).
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