Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed (ab7006)
Key features and details
- Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Overview
-
Product name
Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Mouse, Rat more details -
Immunogen
Full length native Human IgG (purified).
-
Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
IHC-P |
1/50 - 1/200.
|
|
IHC-Fr |
1/50 - 1/200.
|
|
Immunomicroscopy |
1/50 - 1/200.
|
|
Flow Cyt |
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
|
Notes |
---|
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/200. |
IHC-Fr
1/50 - 1/200. |
Immunomicroscopy
1/50 - 1/200. |
Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab7006 has been referenced in 6 publications.
- Asashima H et al. Prior cycles of anti-CD20 antibodies affect antibody responses after repeated SARS-CoV-2 mRNA vaccination. JCI Insight 8:N/A (2023). PubMed: 37606046
- Alexandru N et al. Intravenous Administration of Allogenic Cell-Derived Microvesicles of Healthy Origins Defend Against Atherosclerotic Cardiovascular Disease Development by a Direct Action on Endothelial Progenitor Cells. Cells 9:N/A (2020). PubMed: 32059493
- Tiwari A et al. Controlling the Effective Oxygen Tension Experienced by Cells Using a Dynamic Culture Technique for Hematopoietic Ex Vivo Expansion. Curr Protoc Stem Cell Biol 44:2A.11.1-2A.11.13 (2018). PubMed: 29512129
- Tiwari A et al. Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds. Curr Protoc Stem Cell Biol 36:1C.15.1-1C.15.16 (2016). PubMed: 26840223
- Alvarez-Cienfuegos A et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). PubMed: 27345490
- Tiwari A et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). PubMed: 24510768