Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006)

Overview

  • Product nameGoat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Mouse, Rat more details
  • Immunogen

    Full length native Human IgG (purified).

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.60
    Preservative: 0.05% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesCan be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

References for Goat F(ab')2 Anti-Human IgG H&L (Phycoerythrin) preadsorbed (ab7006)

This product has been referenced in:
  • Alvarez-Cienfuegos A  et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). Read more (PubMed: 27345490) »
  • Tiwari A  et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). Read more (PubMed: 24510768) »

See all 2 Publications for this product

Product Wall

Since there are more than one antibody attached to each phycoerythrin (PE) molecule, the antibody concentration is irrelevant in this case. It only takes one active antibody for a PE-antibody conjugate to bind to its antigen. Other antibody molecule...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"