Goat F(ab')2 Anti-Mouse IgG - Fab' (TRITC ), pre-adsorbed (ab51379)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - Fab' (TRITC ), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBinds all mouse Igs. No cross reaction is observed with the Fc fragment of mouse IgG. No reactivity is observed with human lambda or kappa light chain, IgG, IgA, IgM, IgD, and IgE, bovine IgG and IgM, or horse IgG as demonstrated by ELISA.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Purified mouse IgG, Fab fragment.

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe F(ab')2 fragment of the antibody is obtained from pepsin digested antiserum by immunospecific methods of purification. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to the Fab fragment of mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesF/P Molar Ratio: 1.0 to 5.0
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.
IHC-P 1/100.
ICC/IF 1/32.

References for Goat F(ab')2 Anti-Mouse IgG - Fab' (TRITC ), pre-adsorbed (ab51379)

ab51379 has not yet been referenced specifically in any publications.

Product Wall

Thank you for calling Abcam yesterday.

I have talked to the lab about ab51379 and they have told me that we have not tested theantibody with the variable region only. As this is a polyclonal antibody raised against purified mouse IgG, Fab fra...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"