Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgGF(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG F(ab)2 fragment

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)

ab5886 has not yet been referenced specifically in any publications.

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Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"