Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • Conjugation
    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab98758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.
The user will have to verify the excitation laser and emission detection filters of their instrument.
Dylight 550 properties: Excitation at 562 nm, emission at 576 nm
PE properties: Excitation at 488 nm, emis...

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In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttp://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those col...

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Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your las...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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