Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed (ab5883)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c), anti-Pepsin, anti-Goat IgG F(ab’)2 or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISA, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG F(ab)2 fragment

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (FITC), pre-adsorbed (ab5883)

ab5883 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"