Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed (ab98762)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICCmore details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000. by chemiluminescent detection. Use at 1/2000 - 1/20000 by colorimetric detection.
ELISA 1/10000 - 1/100000. as primary
IHC-P 1/200 - 1/2000.
ICC Use at an assay dependent dilution.

References

ab98762 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for the time taken giving me furthrt infotmation.

I can confirm that the Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (HRP), pre-adsorbed could overcome the cross-reactivity with the human Fc. However w...

Read More

Vielen Dank für Ihre Anfrage.
Wir haben jetzt eine Bestätigung von Labor:
Wir haben einen Antikörper, der selbst ein F(ab)2 Fragment ist (gekoppelt an HRP und auch nur F(ab)2 Fragmente bindet:
ab98659 (Goat F(ab')2 polyclonal Secondary A...

Read More

Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody...

Read More

Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up