Goat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed (ab98754)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAntiserum has been cross adsorbed against human and rat immunosorbents.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Human, Rat more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1,000-1/30,000 dilution.
WB 1/1000 - 1/30000. Colorimetric: Use 1/1,000-1/10,000 dilution; Chemiluminescent: Use 1/1,000-1/30,000 dilution.

References for Goat F(ab')2 Anti-Mouse IgG - (Fab')2, pre-adsorbed (ab98754)

ab98754 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.

I confirmed with the lab and am happy to forward their confirmation that ab98754 will detect both the (Fab) and the (Fab’)2 fragments of mouse IgG.

Please note that ab98754 itself is also a (Fab')2...

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Thank you for this information.

I have noted that this primary/secondary antibody combination has been used and reviewed by another customer. Their Abreview includes a detailed protocol which you may wish to review. This review is at:http://...

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Thank you for contacting us.

Indeed, a mouse-on mouse staining can be quite tricky; however, it is not impossible. The background will depend on the fixation method ( perfusion fixed tissue vs. normally fixed tissue), on the tissue itself ( ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"