Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed (ab5884)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG F(ab)2 fragment

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.7 moles Texas Red per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

References for Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed (ab5884)

ab5884 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your e-mail and for providing more information of your protocol. I have placed an order for your -one vial of replacement vial of ab5884. For your information, the new order number is: 40004. Please do let me know how you are getting ...

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Thank you for your quick reply and your answers to our questions. We can offer you a replacement vial - free of charge. Please do let us know if you would like to try and test it. We are looking forward to hearing from you soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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