Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200.
1/50 - 1/500.
1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 650), pre-adsorbed images
DyLight®-Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - Fc (DyLight® 650), pre-adsorbed(ab98739)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 650), pre-adsorbed (ab98739)
has not yet been referenced specifically in any publications.
Publishing research using ab98739? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
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