Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed (ab5879)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Mouse IgG F(c) whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/1000.
WB 1/500 - 1/2000.
Dot blot Use at an assay dependent dilution.
ELISA 1/2000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed (ab5879)

This product has been referenced in:
  • Yoshikawa T  et al. Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection. PLoS One 5:e8729 (2010). WB . Read more (PubMed: 20090954) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"