Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed (ab5879)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Mouse IgG F(c) whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/1000.
WB 1/500 - 1/2000.
Dot blot Use at an assay dependent dilution.
ELISA 1/2000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

References

This product has been referenced in:
  • Pu J  et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett 12:585-590 (2016). IHC-P ; Human . Read more (PubMed: 27347184) »
  • Li H  et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). Read more (PubMed: 26446566) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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