SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse or Human Serum Proteins.
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesR-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed
Absorption Wavelength: 490 nm, 545 nm and 565 nm
Emission Wavelength: 580 nm
Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
Use at an assay dependent dilution.
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
References for Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed (ab5881)
has not yet been referenced specifically in any publications.
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