Goat F(ab')2 Anti-Mouse IgG - Fc (TRITC ), pre-adsorbed (ab5876)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - Fc (TRITC ), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Mouse IgG F(c) fragment

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesTetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG F(ab’)2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.

References for Goat F(ab')2 Anti-Mouse IgG - Fc (TRITC ), pre-adsorbed (ab5876)

ab5876 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"