Goat F(ab')2 Anti-Mouse IgG - H&L (Biotin), pre-adsorbed (ab5868)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG - H&L (Biotin), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
Minimal
cross-reactivity
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Mouse IgG whole molecule.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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|
WB |
1/200 - 1/10000.
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ELISA |
1/20000 - 1/100000.
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|
IHC |
Use at an assay dependent dilution.
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|
Immunomicroscopy |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/200 - 1/10000. |
ELISA
1/20000 - 1/100000. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab5868 has not yet been referenced specifically in any publications.