Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab5870)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG whole molecule.

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/10000.
ELISA 1/60000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab5870)

This product has been referenced in:
  • Ulasov IV  et al. Survivin-driven and fiber-modified oncolytic adenovirus exhibits potent antitumor activity in established intracranial glioma. Hum Gene Ther 18:589-602 (2007). WB . Read more (PubMed: 17630837) »

See 1 Publication for this product

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The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Pl...

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Thank you for your question. Both antibodies are only a F(ab)2 Fragment of antibody itself (therefore these antibody will not give F(c) mediated binding), but they different in the immunogens used and therefore epitopes recognized: Ab5887 was raised ag...

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Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I...

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Thank you for your clarification and for sharing more details with us. Certainly, we can offer you a replacement vial - free of charge. Would you be so kind to confirm your Purchase Order Number, or Abcam Order Number. We look forward to hearin...

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Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the ...

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Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"