Goat F(ab')2 Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7002)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Cow, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7002)

This product has been referenced in:
  • Schulz U  et al. Interleukin-10 Promotes NK Cell Killing of Autologous Macrophages by Stimulating Expression of NKG2D Ligands. Scand J Immunol 72:319-31 (2010). Flow Cyt . Read more (PubMed: 20883317) »

See 1 Publication for this product

Product Wall

I have issued a free of charge replacement for one vial of ab91532. To check the status of the order please contact our Customer Service team and reference this number. Please be aware this product is currently out of stock. Expect delivery around Dece...

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Thank you for contacting us. The product ab7002 is not an isotype control, but a secondary antibody that binds to antibodies raised in mouse (such as ab45104). I am not certain which ED9-antibody (ab34086 Anti-SIRP alpha antibody [ED9] ??) you ar...

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Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy t...

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Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit t...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of ...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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ab7002 has minimized cross-reactivity with Bovine, Horse, Human, Rabbit and Swine serum proteins. Ab7000 has minimized cross-reactivity with Human serum proteins. Since rat IgG's are not on the list of either product, the antibodies (anti-Mouse) may q...

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This product is 500 kDa if only one antibody is attached and the recommended amount is 1 microgram per 1 X 10(6) cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"