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Product name Goat F(ab')2 Anti-Mouse IgG - H&L (TRITC ), pre-adsorbed See all IgG secondary antibodies
Target species Mouse
Specificity Assay by immunoelectro- phoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Tested applications Suitable for:
ICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
Minimal cross-reactivity Cow, Horse, Human, Rabbit, Rat, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Mouse IgG whole molecule.
Conjugation TRITC. Ex: 547nm, Em: 572nm Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength: 550 nm
Emission Wavelength: 570 nm
Fluorochrome/Protein Ratio: 2.9 moles TRITC per mole of Goat IgG F(ab')2
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Use at an assay dependent dilution.
1/500 - 1/2500.
1/10000 - 1/50000.
References for Goat F(ab')2 Anti-Mouse IgG - H&L (TRITC ), pre-adsorbed (ab5867)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"