Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed (ab6005)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed
    See all IgG + IgM + IgA secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Mouse IgG, IgA and IgM whole molecules

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000 - 1/20000.
Dot blot Use at an assay dependent dilution.
WB 1/5000 - 1/20000.
ELISA 1/1.2e+006.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed (ab6005)

ab6005 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The molecular weight is approximately 150 kDa. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"