Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed (ab6005)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed
    See all IgG + IgM + IgA secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Mouse IgG, IgA and IgM whole molecules

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000 - 1/20000.
Dot blot Use at an assay dependent dilution.
WB 1/5000 - 1/20000.
ELISA 1/1.2e+006.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References

ab6005 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. The molecular weight is approximately 150 kDa. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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