Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed (ab5999)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
See all IgG + IgM + IgA secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISAmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG, IgA, and IgM whole molecules
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/500 - 1/2500.
|
|
ICC/IF |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
|
Notes |
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Flow Cyt
1/500 - 1/2500. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab5999 has been referenced in 4 publications.
- Khaled SAA et al. Quantitative Assay of Mutated Nucleophosmin in Acute Myeloid Leukemia. J Hematol 8:111-120 (2019). PubMed: 32300454
- Hutchinson CV et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). PubMed: 24618035
- Mosiolek M et al. Rumex acetosa Y chromosomes: constitutive or facultative heterochromatin? Folia Histochem Cytobiol 43:161-7 (2005). PubMed: 16201317
- Xia B et al. Versatile fluorescent derivatization of glycans for glycomic analysis. Nat Methods 2:845-50 (2005). PubMed: 16278655