Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed (ab5999)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
    See all IgG + IgM + IgA secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, ELISAmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Mouse IgG, IgA, and IgM whole molecules

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500 - 1/2500.
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Hutchinson CV  et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). Read more (PubMed: 24618035) »
  • Mosiolek M  et al. Rumex acetosa Y chromosomes: constitutive or facultative heterochromatin? Folia Histochem Cytobiol 43:161-7 (2005). Read more (PubMed: 16201317) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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