Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed (ab6006)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (HRP), pre-adsorbed
    See all IgG + IgM + IgA secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Mouse IgG, IgA and IgM whole molecules

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/120000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Images

  • Ab6006 was used at dilution 1/3000 with the primary antibody ab2861 in WB. See the review on ab2861.

References

This product has been referenced in:
  • Bassagañas S  et al. Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of a2ß1 integrin and E-cadherin function. PLoS One 9:e98595 (2014). WB . Read more (PubMed: 24878505) »
  • Wang Y  et al. Comparison between the efficacies of curcumin and puerarin in C57BL/6 mice with steatohepatitis induced by a methionine- and choline-deficient diet. Exp Ther Med 7:663-668 (2014). WB . Read more (PubMed: 24520264) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I can confirm that ab6006 is suitable for use with antibodies ab119484, ab80039 and ab110304.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Thank you for your inquiry.

I am happy to confirm that ab6006 is a suitable secondary antibody for these two primary antibodies:

ab80039 and ab110304.

ab6006 detects the heavy and light chains of mouse IgG and IgM and IgA....

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Thank you for your enquiry. The originator of this antibody conjugated at a 2:1 ratio. There could be 1 or 2 peroxidase per antibody molecule. Peroxidase molecule randomly bind to Lysines of the antibody. If there are lysines at the antigen binding sit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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