Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    See all IgM secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Mouse IgM mu heavy chain

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesLabel: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
IHC 1/500 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/200 - 1/1000.

Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) images

  • Ab5929 was used with the primary antibody ab8226 in WB. See Abreview on ab8226.

References for Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

ab5929 has not yet been referenced specifically in any publications.

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I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positiv...

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We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cr...

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