Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgM mu chain (FITC)
    See all IgM secondary antibodies
  • Description
    Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (FITC)
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISAmore details
  • Immunogen

    Mouse IgM mu chain

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) images

  • Ab5926 was used at dilution 1/100 with the primary antibody ab2784 in Flow Cyt. See the review on ab2784.

References for Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

This product has been referenced in:
  • Zhong Y  et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . Read more (PubMed: 20846785) »
  • Wang K  et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Human, Rhesus monkey . Read more (PubMed: 20025521) »

See all 2 Publications for this product

Product Wall

Hello and thank you for your patience is trying to troublshooting your Flow Cytometry experiment. I have noticed the secondary you are using is specific to the mu chain of mouse IgM. The primary antibodies are to total IgM, so this is likely the reason...

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Thank you for contacting us.

ab5926 shows less than 1% cross reactivity against other mouse heavy or light chain isotypes in ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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Thank you for your enquiry. Ab5926 has not been tested for cross-reactivity with human IgM, just mouse IgM. We do have several human IgM secondary antibodies (for example ab8506), but unfortunately not a Fab-fragment secondary antibody to human IgM....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"