Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgM mu chain (HRP)
    See all IgM secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Immunogen

    Mouse IgM mu chain

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.3% Potassium phosphate
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
Dot blot 1/5000 - 1/100000.
WB 1/2000 - 1/10000.
ELISA 1/90000.
Electron Microscopy Use at an assay dependent dilution.
IHC 1/500 - 1/5000.

References for Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)

This product has been referenced in:
  • Kuge H  et al. Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids. J Biol Chem 289:26783-93 (2014). ELISA . Read more (PubMed: 25096572) »
  • Oksenberg N  et al. Function and regulation of AUTS2, a gene implicated in autism and human evolution. PLoS Genet 9:e1003221 (2013). Read more (PubMed: 23349641) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. Well done on getting your western blot to work. I have read your comments and have examined your blot images. I would like ot take each of your antibodies one at a time: ab3568 - Cytochrome P450 1A1 antibody (ab3...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"