Goat F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin) (ab5932)

Overview

  • Product nameGoat F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin)
    See all IgM secondary antibodies
  • Target speciesMouse
  • SpecificityAssay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins.
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Mouse IgM mu heavy chain

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesR-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

References for Goat F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin) (ab5932)

This product has been referenced in:
  • Lappalainen J  et al. A protocol for generating high numbers of mature and functional human mast cells from peripheral blood. Clin Exp Allergy 37:1404-14 (2007). Flow Cyt ; Mouse . Read more (PubMed: 17845422) »

See 1 Publication for this product

Product Wall

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"