Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC)
    See all IgM secondary antibodies
  • Description
    Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (TRITC )
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • Tested applications
    Suitable for: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Mouse IgM mu chain

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.

References

This product has been referenced in:
  • Ishikawa M  et al. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci 55:8531-41 (2014). ICC/IF ; Rat . Read more (PubMed: 25406290) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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