Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed (ab6108)

Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG F(ab’)2 fragment

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Goat IgG F(ab’)2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed (ab6108)

    This product has been referenced in:
    • Krelin Y  et al. Association between tumorigenic potential and the fate of cancer cells in a syngeneic melanoma model. PLoS One 8:e62124 (2013). Flow Cyt ; Rabbit . Read more (PubMed: 23626777) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"