Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) (ab102279)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameGoat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP)
See all IgG secondary antibodies - Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Tested applicationsSuitable for: WB, ELISA, ICC, IHC-Pmore details
- ConjugationHRP
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C.
- Storage bufferPreservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab102279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000). | |
| ELISA | 1/10000 - 1/100000. (Primary). | |
| ICC | 1/200 - 1/5000. | |
| IHC-P | 1/200 - 1/5000. |
References for Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) (ab102279)
This product has been referenced in:
- Severino P et al. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer 13:533 (2013). WB . Read more (PubMed: 24209638) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"