Customer reviews and FAQs

Overview

  • Product name
    Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: WB, ELISA, ICC, IHC-Pmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000).
ELISA 1/10000 - 1/100000. (Primary).
ICC 1/200 - 1/5000.
IHC-P 1/200 - 1/5000.

References for Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) (ab102279)

This product has been referenced in:
  • Severino P  et al. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer 13:533 (2013). WB . Read more (PubMed: 24209638) »

See 1 Publication for this product

Publishing research using ab102279? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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