Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) preadsorbed (ab6112)


  • Product nameGoat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins..
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal

    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG F(ab’)2 fragment

  • ConjugationHRP


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/100000.

This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Electron Microscopy Use at an assay dependent concentration.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) preadsorbed images

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.

References for Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) preadsorbed (ab6112)

This product has been referenced in:
  • Hu W  et al. miR-19a/b modulates lung cancer cells metastasis through suppression of MXD1 expression. Oncol Lett 12:1901-1905 (2016). WB ; Human . Read more (PubMed: 27588137) »
  • Noguchi M  et al. Inhibition of homologous recombination repair in irradiated tumor cells pretreated with Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin. Biochem Biophys Res Commun 351:658-63 (2006). WB . Read more (PubMed: 17083915) »

See all 3 Publications for this product

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to gen...

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