Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed (ab102288)

Overview

  • Product name
    Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-P, ICC, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Human, Mouse, Rat more details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ICC 1/200 - 1/2000.
ELISA 1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
WB 1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).

References for Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed (ab102288)

ab102288 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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