Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase) (ab6024)

Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2 or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Immunogen

    Rabbit IgG F(ab')2 fragment

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
WB
ELISA
IHC
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/600 - 1/2800.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.

    Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase) (ab6024)

    ab6024 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"