Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG Fc (FITC)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2.
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Rabbit IgG F(c) fragment.

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 1.9 moles FITC per mole of Goat IgG F(ab')2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Rabbit IgG Fc (FITC) (ab6018)

This product has been referenced in:
  • Roussi S  et al. Mitochondrial perturbation, oxidative stress and lysosomal destabilization are involved in 7beta-hydroxysitosterol and 7beta-hydroxycholesterol triggered apoptosis in human colon cancer cells. Apoptosis 12:87-96 (2007). Read more (PubMed: 17136497) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"