Overview

  • Product name
    Goat F(ab')2 Anti-Rabbit IgG Fc (FITC)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2.
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Rabbit IgG F(c) fragment.

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 1.9 moles FITC per mole of Goat IgG F(ab')2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Rabbit IgG Fc (FITC) (ab6018)

This product has been referenced in:
  • Roussi S  et al. Mitochondrial perturbation, oxidative stress and lysosomal destabilization are involved in 7beta-hydroxysitosterol and 7beta-hydroxycholesterol triggered apoptosis in human colon cancer cells. Apoptosis 12:87-96 (2007). Read more (PubMed: 17136497) »

See 1 Publication for this product

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There are currently no Abreviews or Questions for ab6018.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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