Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab’)2.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/5000 of the reconstitution concentration is suggested for this product.
Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat F(ab')2 Anti-Rabbit IgG Fc (HRP) (ab6023)
has not yet been referenced specifically in any publications.
Publishing research using ab6023? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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