Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485)

Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
  • Minimal
    cross-reactivity

    Human, Mouse, Rat more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. (Colorimetric: /2,000-1/20,000 dilution; Chemiluminescent: 1/5,000-1/25,000 dilution).
ICC Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485)

ab98485 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
HEK293 cells engineered to stably overexpress the human Leptin Receptor (hLR) were stimulated with increasing doses of recombinant human Leptin (rhLeptin). The cells were fixed using formaldehyde, permeabilized, quenched, and blocked using proprietary buffers and then incubated overnight at 4°C with a rabbit anti-pSTAT3 (Y705) antibody (abcam, ab76315, 1/500 dilution). Subsequently, the cells were washed and incubated 30 min at room temperature with a HRP-conjugated goat anti-rabbit F(ab')2 (abcam, ab98485). The cells were washed and TMB substrate was added. TMB conversion was stopped and absorbance was read at 450 nm. The cells were washed and stained using JanusGreen. JanusGreen was eluted and absorbance was read at 630 nm.
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Submitted Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"