Goat F(ab')2 Anti-Rabbit IgG - H&L (TR), pre-adsorbed (ab6010)

Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG - H&L (TR), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • Tested applicationsICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG, whole molecule

  • ConjugationTexas Red ®. Ex: 596nm, Em: 620nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.164% Sodium phosphate, 0.878% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons).
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.

References for Goat F(ab')2 Anti-Rabbit IgG - H&L (TR), pre-adsorbed (ab6010)

ab6010 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"