Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

Overview

  • Product name
    Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation
    Gold 10nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Zhang ZH  et al. Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa. Sci Rep 6:25089 (2016). Read more (PubMed: 27121047) »
    • Occhialini A  et al. Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2. Plant J 85:148-60 (2016). Read more (PubMed: 26662726) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    The F(ab')2 concentration for ab39601, Goat F(ab')2 Anti-Rabbit IgG 10nm gold conjugate, is 10 microgram/ml. The gold particle concentration of the 10nm gold conjugate is approximately 5x10e12 particles per ml.

    Application
    Immunohistochemistry
    Human skin containing 3-4 hair follicles was fixed in glutaraldehyde/paraformaldehyde, embedded in London resin, sectioned at 75nm, subjected to a protocol of potassium periodate antigen retrieval and treated with 2ug/ml of a rabbit anti- KRTAP5 antibody followed by Abcam Goat anti-rabbit IgG 10nm gold. at a dilution of 1/25 The localisation was excellent and specific to the keratin in the cuticle and cortex layers of the developing cuticle and cortex. The binding is to the exocuticle and also the darker layer at the edges of the cuticle cells, the a-layer Reaction also occurs with the cortex (upper right) because the KAPs in the cortex share amino acid sequences. Control (no antibody) was negative.
    GE Rogers
    Biochemistry
    The University of Adelaide' South Australia
    Username

    Abcam user community

    Verified customer

    Submitted Dec 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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