Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab39601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

    ab39601 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry
    Human skin containing 3-4 hair follicles was fixed in glutaraldehyde/paraformaldehyde, embedded in London resin, sectioned at 75nm, subjected to a protocol of potassium periodate antigen retrieval and treated with 2ug/ml of a rabbit anti- KRTAP5 antibody followed by Abcam Goat anti-rabbit IgG 10nm gold. at a dilution of 1/25 The localisation was excellent and specific to the keratin in the cuticle and cortex layers of the developing cuticle and cortex. The binding is to the exocuticle and also the darker layer at the edges of the cuticle cells, the a-layer Reaction also occurs with the cortex (upper right) because the KAPs in the cortex share amino acid sequences. Control (no antibody) was negative.
    GE Rogers
    Biochemistry
    The University of Adelaide' South Australia
    Username

    Abcam user community

    Verified customer

    Submitted Dec 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"