Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed (ab6012)

Overview

  • Product nameGoat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent dilution. PubMed: 21498392
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed (ab6012)

This product has been referenced in:
  • Webb TE  et al. An internal ribosome entry site in the 5' untranslated region of epidermal growth factor receptor allows hypoxic expression. Oncogenesis 4:e134 (2015). WB ; Human . Read more (PubMed: 25622307) »
  • Wang Z  et al. Changes in large pulmonary arterial viscoelasticity in chronic pulmonary hypertension. PLoS One 8:e78569 (2013). IHC (PFA fixed) . Read more (PubMed: 24223157) »

See all 4 Publications for this product

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"