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Product name Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed See all IgG secondary antibodies
Target species Rabbit
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Tested applications Suitable for:
ICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
Minimal cross-reactivity Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Rabbit IgG whole molecule
Conjugation FITC. Ex: 493nm, Em: 528nm Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of Goat IgG F(ab’)2
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Use at an assay dependent dilution.
1/500 - 1/2500.
1/10000 - 1/50000.
Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed images
Immunohistochemistry (Frozen sections) - Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009) Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1 (basement membrane marker), 1:400
Secondary antibody: Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009), 1:100
Nuclei were counterstained with DAPI
F-actin was stained with rhodamine-phalloidin, 1:100
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"