Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed (ab6518)

Overview

  • Product nameGoat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Serum Proteins
  • Tested applicationsSuitable for: ELISA, IHC, ICC/IF, Dot blot, WBmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Rat IgG F(ab)2 fragment

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 1.5% BSA, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/50000.
IHC Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/5000 - 1/50000.

References for Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed (ab6518)

ab6518 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"