Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed (ab6518)

Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Serum Proteins
  • Tested applications
    Suitable for: ELISA, IHC, ICC/IF, Dot blot, WBmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Rat IgG F(ab)2 fragment

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 1.5% BSA, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/50000.
IHC Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/5000 - 1/50000.

References

ab6518 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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