Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(ab')2 fragment
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/5000.
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|
Dot blot |
Use at an assay dependent dilution.
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|
WB |
1/2000 - 1/10000.
|
|
ELISA |
1/20000 - 1/400000.
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|
IHC |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/500 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
ELISA
1/20000 - 1/400000. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab6275 has been referenced in 3 publications.
- Liu B et al. The overexpression of RBM3 alleviates TBI-induced behaviour impairment and AD-like tauopathy in mice. J Cell Mol Med 24:9176-9188 (2020). PubMed: 32648620
- Wu X et al. Hydrogen exerts neuroprotective effects on OGD/R damaged neurons in rat hippocampal by protecting mitochondrial function via regulating mitophagy mediated by PINK1/Parkin signaling pathway. Brain Res 1698:89-98 (2018). PubMed: 29958907
- Mochida GH et al. CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development. Nat Genet 44:1260-4 (2012). PubMed: 23023333