Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)

Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Rat IgG F(ab')2 fragment

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/400000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References for Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)

ab6275 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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