Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(ab’)2 fragment
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.16% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/5000.
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|
Dot blot |
Use at an assay dependent dilution.
|
|
WB |
Use at an assay dependent dilution.
|
|
ELISA |
1/10000 - 1/100000.
|
|
Electron Microscopy |
Use at an assay dependent dilution.
|
|
IHC |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/500 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/100000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab6517 has been referenced in 3 publications.
- Motomura E et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . PubMed: 25330272
- Wang SH et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). PubMed: 23363740
- Engelson EJ et al. An Essential Farnesylated Kinesin in Trypanosoma brucei. PLoS One 6:e26508 (2011). WB . PubMed: 22073170