SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Tested applicationsICC/IF, Dot Blot, WB, ELISA, Electron Microscopy, IHCmore details
Minimal cross-reactivity Cow, Horse, Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.