Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed (ab6267)

Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Rat IgG F(ab’)2 fragment

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
Immunomicroscopy
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Louis ED  et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett 518:49-54 (2012). Read more (PubMed: 22561033) »
    • Ørom UA  et al. MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation. Mol Cell 30:460-71 (2008). Read more (PubMed: 18498749) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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