Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed (ab6115)

Overview

  • Product name
    Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.2 moles FITC per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000. PubMed:17173070
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Giannakara A  et al. Sperm competition-induced plasticity in the speed of spermatogenesis. BMC Evol Biol 16:60 (2016). IHC - Wholemount . Read more (PubMed: 26956948) »
  • Louboutin JP  et al. Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes. Neurobiol Dis 38:313-25 (2010). Read more (PubMed: 20219678) »

See all 3 Publications for this product

Customer reviews and Q&As

I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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