Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed (ab6115)

Overview

  • Product nameGoat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.2 moles FITC per mole of Goat IgG F(ab’)2
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000. PubMed:17173070
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References for Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed (ab6115)

This product has been referenced in:
  • Louboutin JP  et al. Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes. Neurobiol Dis 38:313-25 (2010). Read more (PubMed: 20219678) »
  • Bouvard C  et al. alpha6-integrin subunit plays a major role in the proangiogenic properties of endothelial progenitor cells. Arterioscler Thromb Vasc Biol 30:1569-75 (2010). Read more (PubMed: 20508204) »

See all 2 Publications for this product

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I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"